FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24933737
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21800.09.5863711
CGCGTAT5103.367616E-49.31296351
CGGGCAT100250.08.33846951
CGGGAAT54700.08.0752081
CCGGGAT82250.07.85346131
AAAGGGG275200.07.80880-81
CGGGGAT80750.07.7052511
ACGTGCG41100.07.51495746
GCGTCGT214900.07.405894894-95
CGCGGAT37200.07.4053081
CTCGGAT82950.07.27185771
GTCGGAT30800.07.24778371
TCGGATA29600.07.22190952
CGGGATT48700.07.2170681
AAAAGGG280950.07.14958880-81
CGAAAAT51950.07.13127371
ATCGGAA471650.07.10130994-95
GCGGTAT28850.07.07914351
AAGGGGG283000.07.0641682-83
AATCGTG43150.07.0475555
Produced by FastQC (version 0.11.5)