FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25476077
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG287650.09.657310580-81
CGGGAAT55350.09.0963511
CGGGGTT87600.08.783951
CGGGGAT77500.08.3964911
AGGGGGG421950.08.31098682-83
GCGTCGT215550.08.09972394-95
AAAAAGG408900.07.989803378-79
CGGGTAT24300.07.81866651
GATCGGA514100.07.78081994-95
CGTCGTG209850.07.73112394-95
CGGGCAT100900.07.72025441
GTCGGAT28200.07.57953171
ATCGGAA480350.07.53135294-95
TCGCGTT20250.07.392855630-31
CTCGGTT49600.07.37374351
AAGGGGG291900.07.36931582-83
AGCGTCG248300.07.33752394-95
AGATCGG575500.07.26850194-95
AGAGCGT300100.07.21871194-95
CCGGGAT76850.07.16956951
Produced by FastQC (version 0.11.5)