FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30724606
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333690.10860676293131309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG360700.09.60375280-81
AAAAGGG349400.09.23484178-79
CGGGGAT89150.09.0038421
GGCGAAT40100.08.6465141
CGGGGTT107500.08.4389321
CGCGAGT30500.08.2535091
CCGGGAT96850.07.74854231
GCGTCGT261700.07.687949794-95
CCGGAAT67950.07.5491291
AGCGTCG300200.07.52489994-95
GATCGGA616300.07.46566294-95
TCGCACT54300.07.43606472
CTCGGAT85750.07.42220971
ATCGGAA573850.07.37218794-95
GCGGGAT73200.07.26723961
CGTCGTG249250.07.261906694-95
AGATCGG691800.07.23803894-95
GTCGTGT231600.07.230717794-95
AGGGGGG576850.07.206979882-83
CGAATCG21100.07.2046843
Produced by FastQC (version 0.11.5)