FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25035298
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG287550.010.17257580-81
TAGGGGG111050.09.45007150-51
CCGGGAT77150.08.8034341
AGGGGGG423000.08.46435282-83
TCGCGTT19250.08.14726230-31
CGCGTAT5300.00492319748.0652661
AAAAAGG417100.08.06041778-79
CGCGGAT34500.07.9847431
CGGGGAT70250.07.9102751
CGCGGGT44450.07.9070051
GCGTCGT227500.07.653231694-95
AAGGGGG294550.07.617377382-83
GCGGGAT55950.07.5551311
CGGGTAT26500.07.52758171
CCGGAAT51250.07.50659851
CGTCGTG219650.07.504997394-95
AGAGCGT318600.07.47040794-95
CGGGGTT85500.07.44373231
GCGGGTT42200.07.42819931
TAAAAAA683000.07.400879470-71
Produced by FastQC (version 0.11.5)