FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37810647
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT12950.09.5359231
TAGGGGG155300.09.12704950-51
CGGGGAT106300.08.7575371
CGGGTAT38850.08.557881
CGAATCG24050.08.4939043
GCGTCGT345400.08.2869494-95
CCGGGAT113100.08.0210271
CGTCGTG331900.07.979892394-95
GATCGGA868950.07.979372594-95
AAGGGGG451850.07.939666782-83
AGCGTCG404400.07.90611994-95
AAAAGGG457350.07.875392480-81
AGAGCGT488000.07.83187394-95
GCGGTAT33600.07.7746811
GGCGAAT47800.07.7504321
ATCGGAA808950.07.69910894-95
AAAGGGG435100.07.694232580-81
CGCGAGT34850.07.63210631
GTCGTGT309750.07.591949594-95
CGGGGTT129300.07.56707241
Produced by FastQC (version 0.11.5)