FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429910
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT67200.08.8348581
CGCGGGT42150.08.5639651
GGCGAAT30700.08.5090911
CTCGGAT66750.08.3963321
GTTCGAT23400.08.3219831
CGGGCAT94600.08.2340141
GTCGGAT24350.08.1923631
CGCGAAT9306.6615394E-78.1713891
CGGGAAT51750.07.9848681
AAGGGGG264150.07.927725382-83
AAAAGGG269000.07.837764780-81
CGGGTAT25600.07.7923451
CGCGATT8701.984413E-57.64306641
GATCGGA282900.07.59873694-95
TAGGGGG94750.07.51739850-51
GCGTCGT124700.07.46698694-95
ATCGGAA270050.07.42376194-95
AGCGTCG146650.07.418381794-95
GGGAAAT192900.07.3866561
CGCTAAT11703.589903E-77.30710741
Produced by FastQC (version 0.11.5)