FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24054662
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT68650.010.5853761
CGCGATT8401.4106809E-79.04682351
CGCGAAT7905.6407407E-79.0181941
CCGGGAT74150.08.7753571
AAGGGGG260800.08.64870782-83
AAAAGGG270150.08.63940280-81
CTCGTAT23450.08.5067151
CGGGTAT27200.08.3816161
TCGCGTT18650.08.02701430-31
CGCGAGT22650.07.96839431
GCGGAAT42400.07.61725431
GATCGGA392100.07.511990594-95
GCGGGAT48800.07.4942181
GCGTCGT170300.07.420397394-95
GGGGGGA350850.07.3653952
AGATCGG445850.07.33627194-95
CGCGGAT35100.07.30704981
GGCGAAT28800.07.2563061
AGAGCGT240100.07.222056494-95
ATCGGAA373600.07.19094494-95
Produced by FastQC (version 0.11.5)