FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23553842
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT44500.08.2196212
CGGGGAT64200.08.2121761
CGGGTAT27250.08.0179191
CCGGGAT72350.07.94359641
GATCGGA381600.07.905112794-95
CGGCGAA31350.07.7268581
ATCGGAA359950.07.694299794-95
AGATCGG436400.07.532932394-95
CGGGAAT51100.07.52895071
GCGTCGT162250.07.495454394-95
GCGGGAT53700.07.34131961
CGACGAT30000.07.2829431
CGGGCAT94600.07.28026531
GGCGAAT28100.07.26829341
GTCGATT28300.07.21692751
CTCGCAC63300.07.20340541
CGCGGAT34650.07.12804461
CGCCAAT43400.07.11367371
AGAGCGT225350.07.083117594-95
CGGGATT38300.07.0696372
Produced by FastQC (version 0.11.5)