FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459006
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT70050.09.3569991
CGGGAAT50200.08.8938491
CGCGGAT34550.08.6608031
CCGGGAT74100.08.5891911
TCGCGTT18800.08.34225430-31
CGGGGTT80050.08.0694371
GCGTCGT159000.08.06723894-95
TCGCACT41250.08.0612212
GATCGGA374600.08.00873494-95
TACCGTA17450.07.8979549
GGGGAAT128850.07.74106171
GCGGGAT55700.07.67455051
CGTCGTG154600.07.62079894-95
ATCGGAA353150.07.58041594-95
GGGAAAT196350.07.571451
AGATCGG422350.07.40136294-95
CTCGTAT23750.07.39952371
CGCGTTT16750.07.376032432-33
CCGGAAT45750.07.3711111
CTCGGTT50050.07.3072221
Produced by FastQC (version 0.11.5)