FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25662815
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA20700.08.72396859
CGGGAAT53050.08.4159761
CTCGTAT26100.08.3710391
GTCCGAT25050.08.3427081
CGGGTAT25800.08.1001871
TCGCGTT20700.08.03566530-31
GATCGGA399700.07.9216294-95
GCGTCGT171000.07.84820494-95
CGGGGTT88450.07.84001831
CGGGGAT68250.07.79430531
ATCGGAA376250.07.790343894-95
CCGGGAT79050.07.750861
CGCCAAT47100.07.6639861
GGCGAAT30350.07.51181031
AAGGGGG256400.07.361825582-83
AGATCGG454550.07.352421894-95
TCGCACT48300.07.27776862
CGCGAGT24500.07.17294841
AGCGTCG198700.07.16055994-95
GTCGATT31900.07.1468161
Produced by FastQC (version 0.11.5)