FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25562526
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT79700.08.8198731
CGCGAGT24000.08.7076381
CGGGAAT57950.08.523911
CGGGGAT74550.08.3460811
GTCGGAT27700.08.230391
CGCGTAT6353.2622926E-48.227691
AAAGGGG280850.08.04828180-81
GTCGATT29550.08.0365841
CGGGGTT86700.07.99820851
GATCGGA430700.07.8644594-95
CCGGAAT54100.07.63802241
GCGTCGT179200.07.555453394-95
TAGGGGG104350.07.50858750-51
CGTCGTG175100.07.50175194-95
ATCGGAA406200.07.42070994-95
CGCGGAT34300.07.3390641
AGCGTCG211200.07.310433494-95
AGAGCGT255150.07.205581794-95
CGGGATT41000.07.18235351
AGGGGGG406550.07.142532382-83
Produced by FastQC (version 0.11.5)