FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25759438
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4750.00182681228.999271
AAGGGGG291850.08.98973182-83
AAAAGGG305150.08.81582180-81
CGCATAT18650.08.6588151
CCGGAAT51800.08.5273011
CGGGGAT73450.08.4710631
CCGGGAT78850.08.2523431
AAAGGGG290950.07.891404680-81
TAGGGGG102700.07.58307650-51
CGCGAAT10152.4910605E-67.48707771
CGGGCAT103100.07.4630221
GGGGGGT359350.07.353124614-15
AGATCGG467300.07.31962394-95
GATCGGA411900.07.30645794-95
GCGTCGT179400.07.295434594-95
CGCGGAT33900.07.28554441
ATCGGAA387750.07.283700594-95
TACCGTA21000.07.241159
CTCGTTT45950.07.235541
CCCGTTT55550.07.18213561
Produced by FastQC (version 0.11.5)