FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23597221
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17912367.590876908768197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC289750.044.010638
TCGGAAG309100.041.6141933
ATCGGAA312350.041.611022
CGGAAGA314850.041.2739334
GATCGGA311400.040.7635421
TACTCGA65400.032.9590336-37
ACTCGAT65000.032.8664336-37
GAGCACA397850.032.523619
CTCGTAT60650.031.86892944-45
TCTCGTA65100.029.79785542-43
CGTCTGA210200.029.49358416-17
AAGAGCA452900.029.2056277
ACACGTC212450.029.19005612-13
CACGTCT211400.029.1787214-15
ACGTCTG211950.029.14696514-15
CACACGT224050.027.8867212-13
ATGCCGT82850.027.62873348-49
GAAGAGC474100.027.5692026
GCACACG236450.026.39415710-11
ATTACTC87150.025.66570334-35
Produced by FastQC (version 0.11.5)