FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25508773
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT524590.2056508166817745TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT277290.10870377810802581TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG202750.038.32702340-41
GAATCGG210250.037.55850640-41
AATCGCG39600.037.24337840-41
AGAATCG267800.035.7482638-39
CACTCCG337500.035.0788530-31
GAGCACA701350.034.2485779
AGAGCAC716700.033.5216838
ACTCCGG355150.033.4224632-33
CGGAGAA363700.032.61059636-37
CCGGAGA373400.031.91610534-35
TCCGGAG380150.031.36189534-35
ATCGGAA767750.031.0889842
CTCCGGA383300.031.07320632-33
CGGAAGA773100.030.9717244
TCGGAAG771850.030.9607073
GTCACTC397500.030.07074428-29
ATCGGGG190300.029.66491342-43
GATCGGA804450.029.5700151
AAGAGCA839550.028.6842887
ATCGCGT21650.028.52122142-43
Produced by FastQC (version 0.11.5)