FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325599
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT427600.16884102129232956TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG175900.037.6020440-41
GAATCGG184600.036.29304540-41
AATCGCG32050.034.6051640-41
CACTCCG291800.034.27305630-31
AGAATCG239400.033.33244338-39
ACTCCGG308200.032.4646932-33
GAGCACA642300.031.6880689
CGGAGAA317550.031.3965836-37
AGAGCAC651150.031.1771458
CCGGAGA331700.030.2720334-35
TCCGGAG342500.029.4076134-35
CTCCGGA347850.028.92803432-33
GTCACTC350900.028.72405428-29
CGGAAGA707500.028.5195484
ATCGGAA705650.028.4598622
TCGGAAG710700.028.3444083
ATCGGGG172800.027.72133842-43
GATCGGA732950.027.3081571
ATCGGGT72500.026.40274242-43
CGTCTGA387300.026.2022816-17
Produced by FastQC (version 0.11.5)