FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24933737
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT381350.1529453848013236TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG161700.036.3362840-41
GAATCGG169600.035.21785740-41
AATCGCG32050.034.5309640-41
AGAATCG212350.034.02183538-39
CACTCCG284550.032.36681730-31
GAGCACA607150.031.0660179
ACTCCGG299400.031.0073532-33
CGGAGAA305150.030.17402336-37
AGAGCAC627850.029.9963878
CCGGAGA322750.028.66840434-35
ATCGGAA663300.028.0860442
TCCGGAG332000.028.01272834-35
GTCACTC332800.027.95252628-29
TCGGAAG668350.027.8736613
CGGAAGA673500.027.7517594
CTCCGGA335800.027.67451132-33
GATCGGA690950.026.8501661
ATCGGGG160650.026.07766242-43
AAGAGCA742950.025.45157
CGTCTGA371200.025.41927316-17
Produced by FastQC (version 0.11.5)