FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25476077
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT481750.18909897312682797TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT259410.10182493953052504TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG192150.037.89517640-41
GAATCGG198300.037.24687240-41
AATCGCG39600.036.76354640-41
CACTCCG319000.035.7357330-31
AGAATCG256750.035.24249638-39
GAGCACA676600.034.2797559
AGAGCAC679000.034.081648
ACTCCGG339800.033.56223732-33
CGGAGAA349150.032.4866136-37
TCGGAAG725700.031.725053
CGGAAGA730750.031.5641844
ATCGGAA728900.031.5404622
CCGGAGA361550.031.47752634-35
TCCGGAG374100.030.5548634-35
CTCCGGA377650.030.37454632-33
GATCGGA759850.030.1382141
GTCACTC384550.029.90364528-29
ATCGCGT20200.028.8049342-43
AAGAGCA807450.028.724557
ATCGGGG183700.028.49407642-43
Produced by FastQC (version 0.11.5)