FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30724606
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT672800.218977584285377TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT363330.1182537540107105TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT312140.10159284060469319TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG52550.038.50518840-41
AATCGGG261800.038.4544140-41
GAATCGG272150.037.80354340-41
GAGCACA855000.037.4601449
AGAGCAC868250.036.817368
CACTCCG428750.036.54780230-31
AGAATCG351950.035.7573538-39
ACTCCGG460700.034.23484432-33
TCGGAAG928900.034.178923
CGGAAGA930950.034.133774
ATCGGAA927500.034.128422
CGGAGAA467800.033.65940536-37
GATCGGA962350.032.801061
CCGGAGA481000.032.6665734-35
TCCGGAG490550.032.27268634-35
GTCACTC495600.031.80971528-29
ATCGCGT28300.031.72179442-43
AAGAGCA1009850.031.678397
CTCCGGA497350.031.67385732-33
ATCGGGG241400.029.97695742-43
Produced by FastQC (version 0.11.5)