FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25035298
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT513510.205114394883576TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT273750.10934561274245667TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG200650.038.38468640-41
GAATCGG206700.038.07695840-41
AATCGCG37550.038.07472240-41
CACTCCG331450.035.69017830-31
AGAATCG269450.035.22947738-39
GAGCACA694050.035.00549
AGAGCAC703050.034.3140688
ACTCCGG352400.033.75037832-33
CGGAGAA367850.032.25536336-37
CCGGAGA371650.031.88721834-35
TCCGGAG374900.031.81350334-35
TCGGAAG756000.031.610093
CGGAAGA758550.031.5282444
ATCGGAA756300.031.5225032
CTCCGGA383200.031.09344132-33
ATCGCGT20350.030.8098642-43
GATCGGA784050.030.3235151
GTCACTC393450.030.28950528-29
AAGAGCA821850.029.3423467
ATCGGGG191150.029.03585842-43
Produced by FastQC (version 0.11.5)