FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37810647
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2804340.741679982360524TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG368600.043.26549544-45
CGTATGC376850.042.6145346-47
TATGCCG375250.042.56208848-49
CTCGTAT358250.041.50577544-45
TACTCGA395200.040.20916736-37
TTACTCG408450.039.25366634-35
ACTCGAT395050.038.96197536-37
TCTCGTA379950.037.95389642-43
ATGCCGT436350.036.57510848-49
GTATGCC453000.035.29892346-47
CCGTCTT463500.034.38173752-53
CTCGATC422700.034.1547338-39
ATTACTC475150.033.94329534-35
GCCGTCT471000.033.74338550-51
ATCTCGT433450.033.17616342-43
TGCCGTC484600.033.0561450-51
ACATTAC494050.032.8851632-33
TCGATCT441250.032.53050238-39
GAGCACA1022850.032.153519
AGAGCAC1036950.031.555988
Produced by FastQC (version 0.11.5)