FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429910
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1476380.6301261933998039TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG196650.042.95779444-45
CGTATGC203050.042.18870546-47
TATGCCG204950.041.71647348-49
CTCGTAT191700.041.37866644-45
TACTCGA212650.040.00481836-37
TTACTCG218900.038.949434-35
ACTCGAT214450.038.11859536-37
ATCGGAA451900.038.0922162
GATCGGA462650.037.0709951
TCTCGTA206400.037.06236342-43
TCGGAAG467350.036.924333
AGAGCAC478400.036.4482888
GAGCACA489800.035.9587759
CGGAAGA486400.035.5756844
ATGCCGT243550.034.8805948-49
GTATGCC248900.034.1976446-47
ATTACTC260550.033.27919834-35
CCGTCTT261150.032.72102752-53
CTCGATC238050.032.3542138-39
ACATTAC269900.032.3463132-33
Produced by FastQC (version 0.11.5)