FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24054662
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1644770.683763504970471TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG217750.042.75429544-45
CGTATGC222450.042.1926146-47
TATGCCG224250.041.80107548-49
CTCGTAT214000.040.8178244-45
TACTCGA234600.039.70374336-37
TTACTCG240700.038.96394734-35
ACTCGAT234450.038.3920136-37
TCTCGTA226650.037.4603842-43
ATGCCGT262900.035.62862848-49
GTATGCC271050.034.73250646-47
GAGCACA562400.034.1375929
AGAGCAC564750.033.9198468
CCGTCTT278850.033.8721852-53
GCCGTCT282150.033.17281350-51
ATTACTC285200.033.16749634-35
CTCGATC257150.033.00805338-39
ATCGGAA573350.033.005742
TCGGAAG578800.032.703033
ATCTCGT259050.032.6834442-43
TGCCGTC290150.032.53647250-51
Produced by FastQC (version 0.11.5)