FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23553842
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1421880.60367221619301TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG185300.043.16679844-45
CGTATGC193100.041.5708146-47
TATGCCG192500.041.56471648-49
CTCGTAT182550.040.98093844-45
TACTCGA200300.039.5783936-37
TTACTCG210650.038.2200234-35
ACTCGAT200750.038.16467336-37
TCTCGTA196600.036.9408342-43
ATGCCGT230800.034.749648-49
GTATGCC238050.033.920746-47
CCGTCTT245500.032.5529952-53
CTCGATC223350.032.16563438-39
ATTACTC253850.031.94966134-35
GAGCACA517050.031.7578059
GCCGTCT251850.031.65671250-51
TGCCGTC254500.031.4577350-51
ATCTCGT229400.031.28626642-43
AGAGCAC523100.031.1725758
ACATTAC264950.030.76352332-33
ATCGGAA530450.030.4816782
Produced by FastQC (version 0.11.5)