FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459006
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1308670.5578539857997393TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG177400.042.1973344-45
TATGCCG181700.041.2640748-49
CGTATGC182550.041.13698246-47
CTCGTAT173100.039.77438744-45
TACTCGA190900.038.82765636-37
TTACTCG198750.037.7959634-35
ACTCGAT193250.036.90533436-37
TCTCGTA186650.035.86901542-43
ATGCCGT223650.033.57726748-49
GTATGCC227850.033.1459546-47
CCGTCTT233700.032.07254852-53
ATTACTC242000.031.32570334-35
GCCGTCT238850.031.2914250-51
CTCGATC212600.031.24507738-39
GAGCACA502150.030.5339769
ATCTCGT217600.030.43981642-43
TGCCGTC248950.030.3176550-51
ACATTAC251600.030.2437232-33
AGAGCAC505450.030.1842678
ATCGGAA509750.029.6885152
Produced by FastQC (version 0.11.5)