FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25662815
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1335370.5203521125800111TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG181000.041.7514444-45
CGTATGC186700.040.60412246-47
TATGCCG186850.040.35544648-49
CTCGTAT177050.039.8660144-45
TACTCGA195250.038.54630336-37
TTACTCG203900.037.2488434-35
ACTCGAT198000.036.7874936-37
TCTCGTA192000.035.8588942-43
ATGCCGT232450.032.6023448-49
GTATGCC234800.032.45810746-47
ATTACTC243850.031.3898434-35
CCGTCTT245800.030.6096752-53
CTCGATC224000.030.48173538-39
ACATTAC255100.030.01487532-33
ATCTCGT229350.029.7189242-43
GCCGTCT254250.029.67622450-51
TGCCGTC263150.028.85304550-51
GAGCACA535200.028.7547519
TCGATCT237750.028.73883838-39
AGAGCAC540100.028.4323168
Produced by FastQC (version 0.11.5)