FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25562526
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1775960.6947513715976269TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG223200.043.04062344-45
CGTATGC228800.042.40238646-47
TATGCCG231200.041.89035448-49
CTCGTAT216150.041.71954744-45
TACTCGA240600.040.08597636-37
TTACTCG247700.039.07119434-35
ACTCGAT242900.038.23000336-37
TCTCGTA232450.037.60888742-43
ATGCCGT276300.034.88934748-49
GTATGCC279000.034.6963846-47
CCGTCTT287900.033.58281352-53
CTCGATC263400.033.3250238-39
GAGCACA597750.033.0407529
AGAGCAC598800.033.0066158
GCCGTCT291100.032.94431350-51
ATTACTC297600.032.8790634-35
ATCTCGT267350.032.69940642-43
ACATTAC301350.032.63547532-33
TGCCGTC306600.031.76683850-51
ATCGGAA615000.031.7517472
Produced by FastQC (version 0.11.5)