FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n01_HBLm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n01_HBLm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25759438
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2000700.7766861994427052TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG258000.043.46697244-45
CGTATGC261600.042.95059246-47
TATGCCG265150.042.2412348-49
CTCGTAT251650.041.619344-45
TACTCGA272850.040.7443936-37
TTACTCG282800.039.77272834-35
ACTCGAT273100.039.6374536-37
TCTCGTA267050.038.2409842-43
GAGCACA619850.037.0887959
AGAGCAC627550.036.5050438
GTATGCC308500.036.43638646-47
ATGCCGT308550.036.2380948-49
ATCGGAA627700.036.0054362
TCGGAAG640300.035.429893
CGGAAGA651500.034.864154
CCGTCTT320500.034.76847552-53
GATCGGA648200.034.6851651
GCCGTCT323400.034.44197550-51
CTCGATC297700.034.1682238-39
ATTACTC331600.034.0771334-35
Produced by FastQC (version 0.11.5)