FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39693030
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14918673.7585112549986732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1847520.4654519949724171No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA786290.19809271300276146No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC717670.18080504310202572No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC667960.16828143379328814No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG651930.1642429413929851No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA587540.14802094977380162No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT533050.13429309881357004No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC463800.11684671087090102No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT442160.11139487209719187No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT434280.10940963690602608No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG419340.10564575191160765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT357150.069.1153957
TAGGTTG356100.068.974989
GTAGTAG637950.068.8247455
TAGTAGG356650.068.821456
GTAAGGT214850.068.7927252
GGTAGTA641900.068.7610864
GAGGTAG716500.068.7167362
CAGACTG740250.068.710799
TGTATAG464600.068.7068114
GTTGTAT373550.068.5913512
TATAGTT411650.068.432816
GACTGAT746400.068.4305511
CTATCGG212000.068.31409521
GCTATCG212950.068.1396120
TAGTTAA399800.068.02283518
AGGTAGT648700.067.90133
TAGTAGT181600.067.8983156
TGAGGTA703600.067.827681
TAGCTTA739050.067.802191
ATAGTTA403700.067.5723917
Produced by FastQC (version 0.11.5)