FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758358
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG500671.0521906926717157No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA380030.7986578563445627No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC353310.7425040318530047No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT341610.7179157179850696No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA317380.6669947910602775No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT295400.6208023860331652No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC271060.5696502869267087No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA217740.45759482577813604No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG209350.43996269301301No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA204810.430421586606136No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG189860.39900318555266334No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC182230.38296824240630906No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA169780.3568037545724807No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA148410.3118933043709616No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT119980.2521458032371671No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC114120.23983063065032098No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA112740.23693047055307737No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCAA109050.22917569464088244No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC98900.20784480696912677No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC96870.20357862943477562No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG92950.19534049350637342No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA91720.19275556820230844No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC90990.19122142554217234No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC90090.1893300167831004No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG89870.1888676724197717No Hit
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCAAACTGTAGG78730.1654562351130369No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT76230.16020232189339262No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT75980.1596769305714282No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA75330.1583109131343207No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA74880.15736520875478474No Hit
GCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAAG73470.15440200169890536No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT73430.15431793908739108No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT69740.14656316317519616No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC66090.13889244987451554No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA65890.138472136816944No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCA65330.13729526025574368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63090.13258775401094242No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT61870.13002384435975603No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAAACTGTAGGCACC61400.1290361086744629No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA59840.1257576668254049No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA55570.11678398304625251No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA55140.1158803099724737No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC53120.11163514809100115No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA52380.11007998977798643No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT50310.105729749632121No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA49950.10497318612849223No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48490.10190490080821998No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG48330.10156865036216275No Hit
GTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAGA47640.10011857031354095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT159200.069.620548
AGCTTAT334300.069.533392
AGTAGGT159750.069.490387
GTTGACA168100.069.4222618
GCTTATC334550.069.4186553
TATAGTT172750.069.2287816
TAGGTTG160200.069.1204159
TAGTAGG160250.069.087176
CAGACTG336950.069.0802469
TGTATAG208350.069.05360414
TAGCTTA335850.069.048631
CTTATCA336400.068.995274
TGTTGAC187850.068.9768317
CAGCACG97700.068.965594
CATGGGT106300.068.950862
GTAGTAG279800.068.878675
GGTAGTA280750.068.7952044
AGGTTGT193550.068.7104510
GACTGAT339650.068.6959611
TAGTTTG73850.068.667359
Produced by FastQC (version 0.11.5)