FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5046236
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG532171.0545880137195327No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA382960.7589022788470456No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT335960.6657635512885247No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC331020.6559740765196079No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA282230.5592881506136455No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT267120.5293450405411083No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC247140.4897511729534647No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA222610.44114068386813465No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA189660.37584449082444815No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG188980.37449695178743125No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG180110.35691949405457846No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC163590.32418222215528564No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA161330.3197036365322589No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA123590.2449152199778211No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT108120.21425870688568668No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC106040.2101368227724585No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC103150.2044097818651367No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCAA96430.19109292549932266No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA89010.176388896595403No Hit
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCAAACTGTAGG85900.17022588717610512No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC84350.16715429084172836No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC79600.15774133433315446No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG79120.15679013030702488No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG76510.1516179584149453No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA76310.15122162340405798No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC72750.14416686021026365No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT72060.14279950442270237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69440.13760751578007846No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT64590.12799639176606087No Hit
GCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAAG64110.12704518773993131No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA64020.126866836985032No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT61340.12155594783914189No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT60640.12016877530103626No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT60620.12012914179994752No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAAACTGTAGGCACC59890.1186825190102088No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA59340.11759259773026867No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC58220.11537312166929965No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA57530.1140057658817384No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCA56870.11269786034581024No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA54330.10766440570754122No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA52990.10500896113459617No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT51030.10112487802790039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT187650.069.511678
AGCTTAT386750.069.507142
GCTTATC387000.069.444153
AGTAGGT188200.069.420117
TAGGTTG188100.069.382589
CTTATCA387300.069.318064
GTTGACA187450.069.3098918
TAGTAGG188250.069.279776
CAGACTG387050.069.16479
TGTTGAC208250.069.1261117
TATAGTT204350.069.0210616
TAGCTTA388500.069.00691
GTAGTAG329750.068.975275
GACTGAT388950.068.970811
TGTATAG247750.068.9512314
GTTGTAT197250.068.9320112
CATGGGT116500.068.8632052
CAGCACG112000.068.848594
TTGGCGA94500.068.7778417
GGTAGTA332000.068.7503054
Produced by FastQC (version 0.11.5)