FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942398
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA327451.685802806633862No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT201021.034906337424153No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC148420.7641070470624455No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA130620.6724677434799665No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC95130.4897554466180463No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC93060.4790985163699715No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCA86330.44445062237502303No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA71260.36686611085884563No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA64780.33350528573443755No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA63100.3248561829244058No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT62980.3242383898665464No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCAA62200.3202227349904602No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCA57970.2984455297009161No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG57870.2979307021526999No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG46980.24186598215195856No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA42490.21875022523705234No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC42190.2172057425924038No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG39350.20258464022306447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA37440.19275143405213557No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG35120.1808074349335203No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC32920.16948122887276448No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT29860.15372750589734957No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA29010.1493514717375121No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT28730.1479099546025068No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA28400.14621102369339342No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC27890.14358540319749094No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT26110.13442147283924305No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA26060.13416405906513496No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA25920.1334433004976323No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA25060.12901578358297322No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC24560.12644164584189235No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGAACTGTAGGCACCATCAA24000.12355861157188176No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC22310.11485802600702842No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA19660.10121509597929981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG31900.069.4452311
CTAGCGG31550.069.2172912
CATGGTC30300.069.194176
GTCTAGC32200.069.01559410
AGCGGTT31800.069.0032914
GTAGGTT22100.068.885278
AGTAGGT22100.068.885277
GTACAGG6050.068.83684510
AGCTTAT40200.068.783832
GTTGTAT22900.068.6183412
GCTTATC40300.068.52633
ATGGTCT32300.068.4768757
CATGGGT38700.068.284372
TAGGTTG22300.068.267479
GCGGTTA32200.068.2565515
GGTAGTA46650.068.2020654
AGGTTGT27150.068.0602410
ACATGGT30700.067.9505845
CAGCACG18750.067.949364
TGGGTGG38950.067.935954
Produced by FastQC (version 0.11.5)