FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7088422
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1312161.8511313237276223No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA651710.9194006790227782No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT616910.8703065364900678No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCA613760.8658626701401243No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC571670.8064841511975445No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC407150.5743873601204894No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA390610.55105353490523No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG308580.43532961214780946No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT283840.40042762691047457No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA261810.36934877748531336No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA252450.3561441460454809No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC195850.27629562686871634No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA192200.27114638490767057No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA191170.2696933111488001No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA189280.26702699133883395No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCA180630.2548239932667666No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC180320.25438666038788327No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCAA175240.2472200441790853No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT166250.23453739069146845No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC152040.214490615823945No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA150240.21195126362397723No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC147130.20756382732292183No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG139200.1963765701308415No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTAACTGTAGGCACCAT135230.19077588777869037No Hit
GAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAACTGT120960.1706444678378347No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT120460.16993909222673256No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC119880.16912085651785405No Hit
CCCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATC116960.1650014629490174No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA116490.1643384098745814No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG115590.16306873377459752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA106450.15017446760365002No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC102190.14416466739705958No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG93070.1312986162505562No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA87060.12282000140510822No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA85510.12063333701069151No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTTAACTGTAGGCACCATC84880.11974456374070279No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA83130.11727574910184524No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCAC71200.10044548702094769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT153000.069.22972
GTAGGTT89600.069.2154858
TAGGTTG89800.069.0223549
CATGGTC118750.068.710096
CTAGCGG126600.068.642112
AGTAGGT90600.068.6055457
GCTTATC154400.068.533483
AGGTTGT108950.068.5190210
CACATGG119500.068.308164
GGTGAGT81850.068.2459817
GGTAGTA163000.068.20324
GTCTAGC128350.068.1970210
TCTAGCG128300.068.1690311
CATGGGT185000.068.001942
CGTTAGT82200.067.9630665
GTTGTAT95800.067.9508712
ATGGGTG185150.067.927463
ACATGGT120500.067.799385
ATGGTCT128800.067.795247
TATAGTT91950.067.7890816
Produced by FastQC (version 0.11.5)