FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647302
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA788071.185548663202003No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC495030.7447081537742681No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG441480.6641491540477625No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA412760.6209436550347796No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT348930.5249197343523734No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCA341830.514238709178551No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA314360.47291367234405773No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC310770.46751298496743493No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA296910.4466624203323393No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC291340.4382830808649886No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT282160.424472966626159No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC265980.40013226418778625No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC249630.3755358188931389No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA248400.3736854441095049No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC245730.36966877689625055No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG187140.28152775366607385No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG186510.28058000072811495No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA182510.2745625217569474No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA172990.2602409218055686No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA157610.2371037151614294No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA153320.23064996896485218No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT146710.22070608496499783No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA136990.20608361106506068No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA135250.2034660077126028No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTAACTGTAGGCACCAT122180.18380389517431284No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT116960.17595108511693916No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC116020.1745369775587148No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA110860.16677442968590867No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA108780.16364534062090152No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA107080.1610879120581553No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC103260.1553412196406903No Hit
CCCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATC99490.1496697457103649No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAACTGTAGGCACCATC95280.14333634909321105No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT94360.1419523289298425No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC93920.1412904062430141No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA91020.1369277339889176No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT88730.13348272727792418No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA87690.13191818274542064No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA85760.12901474914183228No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCA84440.127028981081347No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG82840.12462198949287998No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAACTGTAGGCACCATCAA82800.12456181470316828No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA81590.12274152731439011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80290.12078584664876066No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTTAACTGTAGGCACCATC78740.11845407354743323No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT75150.11305338617081034No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG75130.1130232987759545No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC74590.11221093911484689No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC72360.10885619458842098No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT72300.10876593240385346No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA71700.10786331055817834No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA71450.10748721812248038No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT66920.10067242318763311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT178000.069.5050668
GCTTATC378250.069.362873
AGCTTAT378500.069.34482
AGTAGGT179350.069.27467
TAGGTTG178450.069.251349
CTTATCA380050.068.933054
CAGACTG380700.068.865869
GGTAGTA308000.068.853614
AGGTTGT211750.068.8236410
TAGTTTG80350.068.820579
TAGTAGG179900.068.7951056
CAGCACG106700.068.787654
TCTAGCG115250.068.7824411
CTAGCGG114400.068.77341512
GTTGACA159000.068.7691418
CATGGGT133650.068.665752
CATGGTC107800.068.6376956
TATAGTT182050.068.6134816
GTAGTAG308200.068.559085
TTGGCGA92600.068.52621517
Produced by FastQC (version 0.11.5)