FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7227053
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT1511222.091059799893539No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC1489622.0611720987794055No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG1187591.6432562484321063No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA963131.3326732210210719No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG854451.182293806341257No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG618970.8564625166025488No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA479540.6635346385310859No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC393080.5439008126825693No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG331420.45858249552064995No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA312260.4320709976805207No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC309490.4282381767506064No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT304380.4211675215333276No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC268980.3721849002629426No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA241620.3343271455183738No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC227970.3154397788420813No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA220560.3051866369320939No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA199010.2753681203112804No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA198840.27513289303399324No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG194580.26923837420315033No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTAACT193130.26723202389687745No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA191470.26493509871866167No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG182990.2532014086516316No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT179000.24768048608471532No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA124550.1723385728595044No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT112410.1555405778814684No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG111320.15403235592709782No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG110840.15336818479122818No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT106340.14714158039245043No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA106250.14701704830447487No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA106230.14698937450714697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104920.14517674078216944No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC100460.1390054839780475No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC98650.13650100531987244No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA96580.13363676729643464No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC94050.13013603193445517No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC92400.12785294365490332No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG91880.12713342492437787No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT84960.1175582910489241No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA83790.11593937390524188No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA83690.11580100491860236No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT78910.10918696735723399No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT78240.1082598951467493No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA77450.1071667801522972No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC76810.10628121863780436No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG76010.10517426674468831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT892450.069.847972
AGTAAGG891650.069.772221
GGTCAGC894850.069.637166
AAGGTCA894300.069.636944
GTCAGCT894200.069.597887
AGGTCAG895200.069.586485
TAAGGTC896150.069.57523
GCTATCG890450.069.48846420
AGCTTAT445200.069.446642
CTATCGG890450.069.43880521
AGCTATC891450.069.4021819
AAGCTAT892650.069.3951518
GCCCATA881150.069.39358528
GCTTATC446050.069.345693
GTAGGTT197950.069.325258
AGCTAAA897400.069.279510
TAGGTTG198300.069.238189
CTAAATA899350.069.1837712
GGCCCAT884250.069.1789827
CCCATAC883150.069.1581329
Produced by FastQC (version 0.11.5)