FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730742
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT831801.2358221426404399No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC760101.1292959973803778No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG491930.730870385464188No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA462880.687710210850453No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC432250.6422025981682258No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG381470.566757721511239No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA361410.5369541723631659No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT356500.5296592857072816No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG355230.5277724209307086No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC352950.5243849786546565No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA348330.5175209508847612No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC294820.438020057818291No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG252680.3754118045231863No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA220220.32718532369833814No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG218100.32403559666972825No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG210740.3131006952873844No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT205960.30599895227004686No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA199710.2967132004168337No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC187310.2782902687400587No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA168680.25061129961600076No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC158700.23578381105678986No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA148960.2213128953687424No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA146600.21780659546896908No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT138870.206321977576915No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA135340.2010773849302202No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC128020.19020191235973688No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT121530.18055958763536026No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC108680.1614680818251539No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA108150.16068065006800142No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA106040.15754578024235663No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA103050.15310347655577944No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT101380.15062232366060088No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG98250.14597201913251168No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC97130.14430801240041588No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC95380.1417080018815162No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT93120.13835027401139427No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA92990.13815713037284744No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA92000.1366862672792985No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA91820.13641883762592594No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT89290.13265996527574525No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA88910.13209539156306985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85100.12643479723335108No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG79970.11881305211223368No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA79470.11807019196397664No Hit
GTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT76610.11382103191594627No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTAACT75710.11248388364908356No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA72260.10735814862610987No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA68110.1011924093955763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT427100.069.773352
AGTAAGG427350.069.603041
AAGGTCA428850.069.398854
TAAGGTC429100.069.374733
GCTTATC459500.069.324883
GTCAGCT429150.069.295227
AGCTTAT460000.069.279962
GTAGGTT211600.069.235748
GCTATCG427900.069.1848320
GGTCAGC430200.069.181076
AGTAGGT211850.069.1705557
AGGTCAG430650.069.108795
CTATCGG428050.069.1038721
GTTGACA252250.069.0710918
TAGGTTG211900.069.055149
GCCCATA421900.069.0139528
GGTAGTA383850.069.0088964
AGCTATC429700.068.9586519
AAGCTAT430350.068.9353118
CAGCACG115550.068.882044
Produced by FastQC (version 0.11.5)