FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5253885
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT965591.8378590319354156No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC856341.629917670447678No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG612691.1661656088780017No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA597141.1365684631467952No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG443570.8442704779415614No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC382070.7272142424129953No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA375720.715127948175493No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC318410.6060467634902553No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT304670.5798946874550928No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG301720.5742797948565681No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC297210.5656956709178066No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA279030.5310927056835085No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC239710.4562528490821554No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG216490.41205698259478457No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG204730.3896735463376149No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA191480.3644541134798345No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC162620.3095233336854537No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA160740.30594502924978373No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA154410.293896802080746No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA149940.2853888122789136No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA137820.2623201687893816No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG136250.2593319039149125No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT133740.2545544868226084No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT123560.23517834897414008No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT120710.22975379171793825No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC114640.21820043643893994No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA113310.21566897638604574No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA108400.20632351107799274No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT106130.2020028988072636No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA104750.19937627108320793No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG104270.19846266144005817No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC99450.18928849794009575No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA98320.1871377085718473No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT96760.1841684772316105No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA96650.18395910835505536No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC95900.18253159328763383No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC95050.18091374287788942No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG94070.17904845652312526No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA93660.17826808161960148No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTAACT93100.17720220370259343No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC92070.17524174967666784No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA83550.15902517851075917No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG81340.15481876744542372No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA80980.15413356021306138No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA78950.1502697527639071No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT78160.14876610355955638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72760.13848799507412135No Hit
GTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT72550.13808829085524332No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA72270.1375553518967393No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG65080.12387024078372481No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG64980.12367990544140194No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC64940.12360377130447278No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA57030.10854824572673365No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA55000.10468443827757937No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA54550.10382792923712644No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA54330.10340919148401612No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA53100.10106806677344479No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG52810.10051609428070847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT520200.069.8764342
AGTAAGG519450.069.823671
GTCAGCT519800.069.674427
GCTATCG518550.069.5452720
CTATCGG518350.069.5315921
TAAGGTC522800.069.522223
GGTCAGC522650.069.5026556
AGCTTAT264900.069.475742
AGCTATC519550.069.469419
AAGGTCA523150.069.469024
AGGTCAG523250.069.395545
GCCCATA512900.069.3715928
AGCTAAA521200.069.3529710
AAGCTAT521100.069.33665518
CTAAATA522100.069.3339712
GCTTATC265800.069.283
TCAGCTA523050.069.228118
GGCCCAT514450.069.2170127
GTAGGTT151600.069.212688
CGAAAAT308350.069.1603238
Produced by FastQC (version 0.11.5)