FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921975
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC389211.3320100274643007No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA270660.9262912927044209No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA229230.7845036319612592No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG200690.6868299694555908No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC200030.6845712232308626No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA157550.5391901025847243No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG150350.5145492346785993No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT148040.5066436228920507No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT114450.3916871294244475No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC114230.3909342140162048No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC105480.36098871482473327No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG104300.3569503503623406No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT94700.3240958598208404No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA86930.2975042565388136No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC84060.28768213280401095No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT67860.23224018001522942No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA63140.2160867221656585No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA63110.21598405188271633No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA62260.21307506053268765No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT61920.21191146399267619No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG61210.20948160062971105No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC59000.20191822311963653No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT57230.19586067642604746No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC52510.17970721857647654No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA48100.16461468698397488No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA44890.15362896670916076No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC44210.15130177362913783No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA42260.1446282052378956No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA41710.1427459167172888No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG41310.14137697961139298No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA38210.1307677170407002No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG37920.12977523763892573No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC35470.12139049786531371No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA35060.11998733733177046No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG34550.11824194252175327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34330.11748902711351056No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG33790.11564096202055117No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA32690.1118763849793376No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC31650.10831714850400842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT90400.069.455018
CAGCACG82300.069.451324
AGTAGGT91050.069.420447
GCTTATC244600.069.41753
AGCTTAT244850.069.374032
GTTGACA139650.069.3501718
TAGCAGC86950.069.19911
TGTTGAC147900.069.19601417
ATATTGG82800.069.1504714
TAGGTTG90650.069.147649
GGTAGTA202300.069.10454
CAGACTG245650.069.0709469
AGTAGTT70950.069.0597847
GAGGTAG224500.069.036382
CTTATCA245600.069.020844
TAGTTTG70900.068.9603969
TTGGCGA78650.068.9325817
TAGTAGT71300.068.92426
TATAGTT115450.068.90568516
AGGTTGT112150.068.903610
Produced by FastQC (version 0.11.5)