FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5752891
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA534990.9299498287035163No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG534290.928733049174754No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC512270.8904566417128362No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC498880.867181387584086No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT446960.7769311116793278No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA440390.7655107666736602No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC388270.67491283947497No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG335560.5832893409591804No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT308400.536078295243209No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG250390.43524203743822015No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC231360.4021630168205864No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA218790.38031313299695757No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT200540.34858995242565866No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA164530.28599533695319446No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA156890.2727150575249905No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT154570.2686823025153788No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC147700.25674048056881316No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG143660.2497179244313859No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA133440.23195294331145852No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG130890.22752038931382498No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA123520.21470943913242924No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA114900.19972566836395822No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA113250.19685754518901888No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA113200.19677063236553585No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC111460.1937460661083271No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT109540.1904086136865795No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC103170.17933591997484397No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC99340.17267839769604534No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT92480.1607539583141763No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG89330.15527845043474664No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA89220.155087242223084No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA87610.15228864930693106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83480.1451096500872344No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT78170.13587950823333866No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC76300.1326289686350741No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT76030.13215963938826583No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT74500.1295001069896857No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA72450.12593668122688229No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG68930.11981801845367833No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC68390.11887935996006181No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG65850.1144641885271249No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA65460.1137862685039574No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA64410.11196109921081418No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA62740.10905821090648163No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG61900.10759807547196705No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA60080.10443444869718548No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA59070.10267880966282866No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT57810.10048860651105679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT480300.069.515652
AGTAGGT219750.069.454517
GTTGACA310350.069.3717318
GCTTATC481350.069.3712843
GTAGGTT218300.069.370758
TGTTGAC328550.069.2463117
CAGCACG128800.069.2430044
TAGGTTG218600.069.19559
GCACGTA129150.069.082456
GGTAGTA391700.069.0120244
GTAGTAG390200.069.008215
GAGGTAG434050.069.0039752
TAGTAGG220050.069.0018166
CAGACTG482550.068.9733669
TTGGCGA113950.068.9276217
CTTATCA483950.068.919034
TGTATAG282050.068.9170614
TATAGTT254250.068.9097516
TAGCAGC144200.068.768371
AAGATGC66750.068.692985
Produced by FastQC (version 0.11.5)