FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297982
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC337971.4707251840963071No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC285451.2421768316723107No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA283521.2337781584015888No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG170080.7401276424271382No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC145910.6349484025549373No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG138700.6035730480047277No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA132200.57528736082354No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA112440.48929887179272946No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC99060.43107387264130004No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA99040.4309868397576656No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT98650.4292896985267944No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT93210.4056167541782312No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT86390.3759385408588927No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA78570.3419086833578331No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC76510.33294429634348743No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC75110.3268519944890778No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT70570.3070955299040637No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA68810.2994366361442344No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT65510.2850762103445545No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT64690.28150786211554313No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA63760.2774608330265424No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG60870.2648845813413682No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG60010.261142167345088No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT58310.25374437223616203No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA57780.25143800081984974No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC57100.24847888277627936No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA55450.24129866987643941No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA50660.22045429424599497No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC49740.21645078159881148No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG48970.21310001557888614No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG47870.20831320697899286No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG46040.20034969812644313No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA43780.19051498227575325No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA42320.18416158177044034No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA35110.1527862272202306No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG33410.14538843211130462No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA33290.14486623480949806No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA32960.1434301922295301No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG31730.13807766988601303No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC29440.12811240470987154No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG27880.12132383978638649No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA27750.12075812604276273No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG26840.11679812983739646No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT25970.11301219939929903No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA25530.11109747595934173No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCT24610.10709396331215824No Hit
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG24330.1058755029412763No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT24090.10483110833766321No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC23780.10348209864132966No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23770.10343858219951244No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA23710.10317748354860916No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA23400.10182847385227561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCC401.3460522E-1069.9995245
GGTTACG455.456968E-1269.9995245
AGCTTAT175750.069.567462
GTTGACA100150.069.3704718
GCTTATC176700.069.3122943
TAGTTTG50600.069.306319
GCACGTA54150.069.229936
CAGCACG54000.069.2277764
AGTAGGT66800.069.21217
GTAGGTT66400.069.207368
GGTAGTA144100.069.131164
GGCTATG19850.069.1179216
CAGACTG177350.069.090229
TGTTGAC107400.069.0544617
GTAGTAG144000.069.033325
CTTATCA177250.068.998484
TATAGTT82500.068.98134616
GGGCTAT20550.068.9776315
TAGCAGC56000.068.943031
GAGGTAG161250.068.7900162
Produced by FastQC (version 0.11.5)