FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474550
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC341640.7635181191404722No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG314530.7029310209965248No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA296750.663195181638377No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT285650.6383882178096122No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA252770.5649059681979194No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC251370.5617771619492463No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC242540.5420433339665441No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG209450.4680917634175503No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT183250.40953838933524045No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA176140.3936485233151937No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG145390.32492652892469637No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA126460.2826205987194243No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT126310.28228536947849503No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC111000.24806963828764902No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA109010.24362226369132092No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT91890.2053614329932619No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT82500.1843760825110905No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC81270.18162720273547062No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA81210.1814931110390989No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA73610.16450816283201664No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC72710.16249678738644108No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA72270.16151344827971528No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG70600.15778122939736958No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC69930.1562838721212189No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC67550.1509649014984747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64270.143634555430155No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG62100.13878490574471175No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT61000.13632655797789722No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA59860.1337788157468349No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT59350.1326390363276754No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA58530.13080644981059547No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA56450.1261579376697098No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG56070.12530869025935568No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT55010.12293973695678895No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA54040.12077192119877976No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA51510.11511772133510631No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA49910.11154194276519426No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA47480.10611122906214034No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA47220.10553016504452961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT409150.069.537032
GCTTATC409000.069.5368653
GTTGACA265050.069.44746418
AGTAGGT197150.069.447077
GTAGGTT196650.069.427888
TAGGTTG196600.069.374339
TGTTGAC283650.069.3218717
GTAGTAG349000.069.3170245
TAGTAGG197600.069.2373356
AGGTTGT233300.069.23231510
GAGGTAG390750.069.201792
GGTAGTA350700.069.18064
CTTATCA410850.069.172644
CAGACTG410000.069.1523069
TTGGCGA102400.069.1460317
CAGCACG114600.069.143824
TGTATAG255350.069.1064914
TATAGTT229300.069.08391616
TAGCAGC124400.069.07361
TAGTAGT97800.069.06856
Produced by FastQC (version 0.11.5)