FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5713798
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1061201.8572585170144273No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC679801.189751545294391No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT615751.077654477809681No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA599931.049967114693239No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA557040.9749032079888019No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG498060.8716793978366053No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC459790.8047011812458192No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC428070.7491864430629154No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG378560.6625365474943287No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT338420.5922855515718266No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG309350.5414087092333331No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA303230.5306977950568081No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC293280.5132838087730788No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT276300.4835662723813477No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT260390.4557213958211333No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA246110.4307292627425751No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA240590.4210684381911996No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA199330.34885727496841856No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA196020.34306428053634375No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA184760.323357598571038No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA163450.28606191538447806No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG161550.28273663157150464No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA154710.27076560984480025No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC153250.26821039175693645No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC149700.2619973614748019No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT145310.254314205717458No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG142310.24906375759171043No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG129860.2272743978698582No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG129060.22587427836965884No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC121980.21348322079289467No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA117970.20646512179814547No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA111630.1953691747590657No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT111050.1943540881214212No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT103740.18156049618834968No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA101390.1774476451565141No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG92520.1619238201980539No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT91830.16071621712913198No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA91470.16008616335404227No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC89860.1572684228598911No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC89670.15693589447859374No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA87310.1528055419530057No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA84920.14862268494616016No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA84410.14773010876478307No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA80710.14125455607636112No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT78060.1366166602319508No Hit
CCACAATGATGGCAATATTTTTCGTCAACAGCAGTTCACCTAGTGAGTGT77810.1361791228881385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75700.13248630770636274No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG75190.13159373152498566No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG72290.12651829833676304No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA70020.1225454592549474No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA69690.12196790996111517No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG68640.12013025311710356No Hit
GAGTTATGATGTGTGTAAATCCTATTCCATTGCTGAAATGCAGTGTGGAA67520.11817008581682448No Hit
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG66880.117049990216665No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG66270.11598239909776299No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT63740.11155452117838259No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCT63570.11125699578459021No Hit
AGCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAAT61890.10831674483417159No Hit
GTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAGA59730.10453642218363338No Hit
CTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGA59660.10441391172736593No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT58580.10252375040209682No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC58230.10191119812075962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT362900.069.475892
GTAGGTT202150.069.407948
GCTTATC363800.069.390613
GTTGACA244350.069.3563118
AGTAGGT203350.069.3425757
TGTTGAC262150.069.305417
TAGGTTG202550.069.219049
TGTATAG261550.069.0866714
TAGTAGG203950.069.0109566
GGTAGTA359150.069.002544
TAGTTTG98100.068.997669
GAGGTAG400950.068.976242
TATAGTT232550.068.9744116
CAGCACG122550.068.921024
ATATTGG122750.068.8846314
TTGGCGA110250.068.8250517
GTAGTAG358800.068.81625
CAGACTG367750.068.787959
CTTATCA367800.068.674024
TGCAGGT39250.068.6590715
Produced by FastQC (version 0.11.5)