FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452406
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC394281.1420441280660503No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC306550.8879314889384389No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG290890.8425718180306719No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA288790.8364891035411246No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA273240.7914480510113816No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT267790.7756619586456517No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC232210.6726033960084649No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG186000.5387547119313314No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC181070.5244748155344418No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT168810.4889633490383229No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG151740.4395195698304313No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT140390.40664394627978284No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA138910.40235708083000665No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT120120.347931268802105No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC110660.3205300882920491No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA107640.3117825655499382No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA107420.3111453287938904No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA92030.26656772117763666No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA82820.23989067334490785No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA81090.23487967521780462No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG75820.21961495837975023No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA74530.2158784337647426No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC74420.2155598153867187No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA69990.20272818434448323No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT68580.19864407604435866No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC66890.1937489391456277No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG60270.1745739058500072No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA58630.1698235954867417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG58360.1690415321952285No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC57920.16776705868313285No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT57330.166058105564641No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA54740.15855609102753268No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG51760.14992442951379414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49180.1424513802837789No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT48390.1401631210234254No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA46970.13605004741620771No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT45740.1324873146437586No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT45150.13077836152526673No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG44400.12860596349328557No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC43240.125245987870488No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC43080.1247825429569987No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA40460.11719363249861112No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG39860.11545571407302617No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA39660.11487640793116452No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA39480.11435503240348904No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA37490.10859093629196567No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA36500.1057233708897505No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT36020.10433303614928255No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT35920.10404338307835174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT116800.069.4856648
AGCTTAT228800.069.437142
AGTAGGT117350.069.398587
GCTTATC229100.069.376763
TAGGTTG117150.069.248199
GTTGACA148000.069.1291318
GGTAGTA203000.069.106564
AGATGCT35450.069.015816
CAGACTG230450.068.977549
TATAGTT132850.068.9706416
TAGTAGG117750.068.962776
CTTATCA230300.068.9544754
TGTTGAC158700.068.92245517
GTAGTAG202850.068.91615
TGTATAG149300.068.84843414
CAGCACG71800.068.784444
GAGGTAG228900.068.779862
TAGTTTG56500.068.7561959
AGTAGTT56500.068.508437
GACTGAT232800.068.5067511
Produced by FastQC (version 0.11.5)