FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343378
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC382101.630552134568132No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA298921.2755944623530646No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC233880.9980464099261834No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG184130.7857460469459047No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA169110.7216505403737681No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG167040.7128171383361968No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA140280.5986230134446939No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC138330.5903016926846629No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT118950.507600566361893No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT113660.48502631671032165No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC111840.477259750667626No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT109150.4657805953627627No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA95290.40663520780684975No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG86980.37117357933717904No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA75960.32414744868305495No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC74750.3189839624678562No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT74460.3177464327138003No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA73410.3132657215353221No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG71590.30549915549262646No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC67490.28800304517666375No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC65030.27750537898708616No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA64080.2734514022065582No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT62640.26730642687607375No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT58960.2516026010315024No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA56770.2422571177163906No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA55100.23513065327062044No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC50490.21545819752511117No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG48520.2070515298854901No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA46050.19651119025611746No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA41310.1762839797932728No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG40010.17073643261991878No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA36400.1553313208539126No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA36320.15498993333555236No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG36300.15490458645596228No Hit
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG33870.14453494058576977No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA32830.14009690284708656No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC30990.13224498992480085No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA30080.12836170690345303No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCT29470.12575862707595617No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT29270.12490515828005554No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA28870.12319822068825431No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT28850.12311287380866426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27780.11854681575059593No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA27000.11521828744658354No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA26360.11248718729970153No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT24740.10557409005290652No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG23640.10088001167545312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT150650.069.518222
GCTTATC151000.069.403443
GGTAGTA147900.069.296144
GTAGGTT68900.069.286038
AGTAGGT69100.069.237447
GTTGACA82700.069.1537318
CAGCACG54350.069.104514
TGTTGAC88850.069.0941517
GAGGTAG165100.068.9884952
TAGGTTG69150.068.984939
TATAGTT88500.068.97192416
CGTAAAT54400.068.967819
TTGGCGA52200.068.9273717
GTAGTAG148350.068.897195
ATATTGG54650.068.84737414
CTTATCA151800.068.8301544
CAGACTG151950.068.799479
TAGTAGT48450.068.777966
TAGTTAA85450.068.73041518
TAGTTTG48500.068.6982659
Produced by FastQC (version 0.11.5)