FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339407
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG354460.8168397202659257No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA319590.7364831185459211No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC235940.5437148439867475No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC221720.5109453895428568No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA189320.4362808097972834No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT184700.4256341937965257No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT179260.4130979186787504No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG177760.4096412251720108No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC157700.3634137106752144No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG107450.24761447819944063No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA106580.24560959596553172No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC97990.2258142644836034No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT85070.19604061107888704No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT79750.1837808714416509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74290.17119850707711906No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC68930.15884658894636988No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA66390.15299325460829094No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA62150.14322233429590725No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG60020.13831382951633714No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA54020.12448705548937908No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA53360.12296611034641369No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA52930.12197519154114836No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA51810.11939419372278286No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC50880.11725104374860436No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA50090.11543051850172155No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT49630.11437046582632143No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC46690.10759534655311198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG45990.10598222291663353No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT43940.10125807512408953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT179350.069.5080648
AGCTTAT401050.069.496972
GTTGACA247050.069.4498218
GCTTATC401600.069.4017943
TGTTGAC262550.069.3216717
AGTAGGT180600.069.298287
TAGGTTG180500.069.181559
GTTGTAT187350.069.13660412
CTTATCA403000.069.117274
TGTATAG232150.069.10644514
TAGTAGG180200.069.0902566
TATAGTT206300.069.0644516
AAGATGC51900.069.0590065
CAGACTG403900.069.0340969
TTGGCGA96600.069.0232817
CAGCACG110600.068.958824
GTAGTAG316050.068.951075
GACTGAT404500.068.94034611
GAGGTAG354550.068.8777542
GGTAGTA318050.068.825624
Produced by FastQC (version 0.11.5)