FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068730
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC153821.4392783958530218No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA107981.0103580885724177No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC97630.9135141710254226No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA96820.9059350818260927No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG85620.8011377990699241No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT64150.6002451507864475No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA63850.5974380807126215No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG60470.5658117578808493No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC51520.4820675006783752No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG46900.4388386215414557No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT45300.42386758114771733No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC44030.4119843178351876No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT40870.3824165130575543No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA34240.3203802644260009No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC32780.3067191900667147No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG29710.2779935063112292No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT26720.25001637457543063No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA26470.24767714951390904No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA25740.2408466123342659No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA25490.2385073872727443No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT25380.23747812824567474No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT24850.23251897111524894No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC24820.23223826410786633No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA21000.196494905167816No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA20110.18816726394879904No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC19790.18517305587005137No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC18850.17637756963873008No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG17310.1619679432597569No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA16820.15738306213917452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16810.15728949313671367No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG15640.1463419198487925No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA14790.138388554639619No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA14220.1330551214993497No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA13660.12781525736154126No Hit
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG13610.12734741234923694No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG13320.12463391127787186No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA13090.12248182422127199No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG12930.12098472018189814No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA12630.11817765010807219No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC11460.10723007682015102No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA11330.10601367978815977No Hit
TCGAGAGGGGCTGTGCTCGCAAGGTTTCTTTAACTGTAGGCACCATCAAT11060.10348731672171643No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT10880.10180307467742088No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGC207.929886E-569.99728410
CTGGTAC150.002225203769.99728465
GTAGGTT32350.069.7809148
AGTAGGT32900.069.571787
TAGGTTG32450.069.565879
AGCTTAT74400.069.536642
GCTTATC74600.069.4909743
TAGCAGC31300.069.45121
CAGCACG29350.069.41084
GGTAGTA71300.069.3689044
TAGTAGG32650.069.363866
GGGCTAT11000.069.3609515
AGATGCT10450.069.337196
GCACGTA29550.069.296386
GTTGACA43150.069.267318
TATAGTT41650.069.1569816
GTCTCGG8300.069.15394611
TGTTGAC46000.069.08427417
GTAGTAG71400.069.0756455
CTTATCA75000.069.0270164
Produced by FastQC (version 0.11.5)