FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6257990
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC538710.8608355078867177No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC492640.7872176209933222No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA469250.7498414027507234No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG447480.7150538751260389No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT425650.6801704700710611No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA407180.6506562011124978No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC353910.5655330225839288No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG315840.5046987930629483No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT287400.45925289110401263No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA227820.36404660282295115No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC227610.36371103181692527No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG217090.34690052237219937No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT196280.3136470336322046No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA168210.2687923758267431No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA165310.2641583000292426No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC150300.24017296288424878No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT148030.23654560010482598No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA127010.20295654035880528No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA118150.18879863981885558No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG113880.18197536269632902No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC112220.17932275379155288No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA111630.17837995906033727No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA108670.17365000583254367No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC104050.16626744369997395No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA103750.1657880565485084No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT102140.16321534550230984No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG92440.14771516093825654No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC92220.14736361036051512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91960.14694814149591162No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA87370.13961351807848846No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT83400.13326962810742748No Hit
GTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTA80040.1279004920110131No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT78910.12609480040715948No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG75300.12032617501785718No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA74680.119335441571495No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG73830.1179771779756759No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA65750.10506568402953663No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC65720.10501774531439009No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT65480.10463423559321763No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG64270.10270070741563984No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC63860.10204554497530358No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA62680.10015995551287235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT260000.069.418368
AGCTTAT503400.069.379782
GTTGACA332000.069.35857418
AGTAGGT262250.069.3566067
TAGGTTG259850.069.3506859
GCTTATC503950.069.33243
TAGTAGG261300.069.215796
TGTTGAC353450.069.208917
GAGGTAG514150.069.202252
TATAGTT304150.069.1818516
TGTATAG340150.069.1529214
GTAGTAG458450.069.1051945
CAGCACG154800.068.988614
GGTAGTA461750.068.9827654
AGGTTGT311400.068.951410
AGTAGTT123100.068.8883747
CTTATCA506600.068.886824
CAGACTG506950.068.844269
GCTACAT66300.068.84412
TAGTAGT123650.068.817196
Produced by FastQC (version 0.11.5)