FastQCFastQC Report
Mon 8 Oct 2018
HTVMCBGX7_n01_AL04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVMCBGX7_n01_AL04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195171
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC299181.3629006578530785No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA208870.9514976282029965No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC173990.7926034008284548No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG148650.6771682023860556No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA142810.6505643523898594No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG131120.5973110978598022No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT104660.4767737911989544No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA93710.4268915724560866No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC92610.4218805733129674No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT79420.3617941381332024No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT73180.33336810663041744No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC71230.3244849717857971No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG66510.3029832300080495No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA64120.29209569550618153No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG51980.23679248678121204No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA50630.23064262419647488No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT48840.2224883619544901No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA48570.22125838943754267No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT47510.21642960844508238No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC43060.19615783918428223No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42610.19410788498936984No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA40800.1858625136720556No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA37380.17028286179072155No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC37290.16987287095173906No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA37160.1692806619620977No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT34180.1557054097380113No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC33920.1545209917587286No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG32180.1465945022050674No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA32110.14627562044141434No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA31190.14208460297626016No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG30710.13989798516835364No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA27440.12500165135199034No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT25630.11675628003467611No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25560.11643739827102306No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA25390.11566297113072285No Hit
TGTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTG23540.10723538166274975No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT22830.10400100948855465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA66350.069.36832418
GTAGGTT68900.069.234638
GCTTATC115700.069.217973
AGCTTAT115600.069.1870042
GGTAGTA141550.069.163794
AGTAGGT69500.069.090137
GAGGTAG157700.069.0501252
AGTAGTT43600.069.03337
CAGCACG45450.069.003374
TAGGTTG69200.068.934479
TGTATAG96000.068.8679714
GTAGTAG142000.068.846015
TGTTGAC70850.068.7678817
TAGTAGG69650.068.748186
AGGTTGT84300.068.7095610
CAGACTG117200.068.652769
TATAGTT85000.068.6409316
TTTGTGC42450.068.5965412
GTTGTAT69400.068.5860912
TAGTTTG43800.068.558279
Produced by FastQC (version 0.11.5)