FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25498540
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19937277.818984930117567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG181700.015.7356723
ATCGGAA185750.015.70381452
CGGAAGA186900.015.4398984
GATCGGA187250.015.2541691
TCGTGTA89250.014.72675114-15
CGTGTAT18000.012.80823314-15
GAGCGTC228900.012.1783279
GTAGGGA90350.012.02499318-19
AGCGTCG115100.011.66046310-11
TAGGGAA94050.011.29914318-19
AGAGCGT252300.010.4983618
GTGTAGG104450.010.2639416-17
AAGAGCG278600.010.15815457
TGTAGGG103300.010.12507716-17
AGGGAAA145900.08.63641420-21
TATTACG12350.08.47309152-53
CGGGAAT20350.08.1605151
GAAGAGC383000.08.116966
GGAAGAG395400.07.9214975
ATTACGA19500.07.6839654-55
Produced by FastQC (version 0.11.5)