FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26470181
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT10203.929017E-109.3069861
AAAGGGG315650.09.25830280-81
CGGGGAT76650.08.2360441
CCGGGAT87150.08.2241071
GCGTCGT200600.08.04329794-95
AAAAAGG438450.08.00413178-79
CGGGGTT90200.07.94601961
CGCGAGT25750.07.9262991
CGGGAAT59700.07.87118341
CTCGGAT76100.07.79658751
CGCGAAC15504.0017767E-117.65574551
GATCGGA478400.07.57858794-95
CGGGATT46750.07.51327561
ATCGGAA454300.07.505335394-95
CGGGCAT101300.07.49703841
AGAGCGT284850.07.405282594-95
AGGGGGG457800.07.35670582-83
AGCGTCG234500.07.325744694-95
GCGGGTT51250.07.3166531
TAGGGGG115800.07.303756750-51
Produced by FastQC (version 0.11.5)