FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27507391
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG332300.010.96786980-81
CGGGGAT76350.010.3200981
TAGGGGG132200.010.09972650-51
AAGGGGG321850.09.08439182-83
AAAAAGG482000.08.91255878-79
CGGGAAT57800.08.86912251
AGGGGGG465450.08.85865982-83
CGGGGTT96100.08.4461241
GGGGGGA456700.08.282172
AAAAGGG355050.08.2107480-81
CCGGGAT88100.08.13552951
AATCGAA40500.07.968035
TAAAAAA778400.07.859128570-71
CGGGCAT106500.07.79961541
CGAATCG20150.07.53660873
GCGTCGT224500.07.45126594-95
GGGGGGT452700.07.354710614-15
GGGAAAA357950.07.339116622-23
GTCGATT28600.07.3024971
GATCGGA529150.07.26877994-95
Produced by FastQC (version 0.11.5)